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KMID : 0355220080330030229
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Journal of Korean Academy Oral Medicine
2008 Volume.33 No. 3 p.229 ~ p.240
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Influences of Animal Mucins on Peroxidase Activity in Solution and on the Surface of Hydroxyapatite
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Lee Sang-Goo
Jeon Eun-Hyoung
Kho Hong-Seop
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Abstract
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Animal mucins have structural characteristics similar to human salivary mucins. Animal mucins have been regarded as suitable substances for saliva substitutes. Since animal mucin molecules in saliva substitutes and host-derived antimicrobial salivary molecules exist simultaneously in whole saliva and the pellicles of patients with dry mouth, interactions may occur between these molecules. The purpose of this study was to investigate the influence of animal mucins on peroxidase activity in solution and on the surface of hydroxyapatite (HA) surfaces. The effects of animal mucins on peroxidase activity were examined by incubating porcine gastric mucin (PGM) or bovine submaxillary mucin (BSM) with either bovine lactoperoxidase (bLPO) or saliva samples. For solid-phase assays, immobilized animal mucins or peroxidase on three different HA surfaces (HA beads, HA disc, and bovine tooth) were used. Peroxidase activity was determined with an NbsSCN assay.
The obtained results were as follows:
1. PGM enhanced the enzymatic activity of bLPO in solution phase. PGM did not affect the enzymatic activity of peroxidase in saliva sample (POS).
2. BSM did not affect the enzymatic activities of both bLPO and POS in solution phase.
3. HA-adsorbed PGM increased subsequent bLPO adsorption in all three HA phases. The activity of POS was increased on both the HA beads and bovine tooth.
4. The peroxidase activities on the HA beads and disc were increased when the HA surfaces were exposed to a mixture of bLPO and PGM.
5. The binding affinity of bLPO to PGM was greater than that of bLPO to BSM.
Collectively, our results suggest that animal mucins affects the enzymatic activity of peroxidase on the HA surfaces as well as in solution. Saliva substitutes containing animal mucins may affect the function of antimicrobial components in natural saliva and saliva substitutes.
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KEYWORD
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Porcine gastric mucin, Bovine submaxillary mucin, Peroxidase, Saliva
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